Primer
Part:BBa_K590083:Design
Designed by: Michael Brasino Group: iGEM11_Washington (2011-09-22)
pGA Prefix, forward primer
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 1
- 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 1
- 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 1
Illegal BglII site found at 16 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 1
- 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 1
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
Does not contain Not1, which makes gibson cloning difficult.
Source
Synthetic